Multiple norovirus genotypes characterised from an oyster-associated outbreak of gastroenteritis.
Int J Food Microbiol
; 103(3): 323-30, 2005 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-15967530
ABSTRACT
The diversity of norovirus (NV) genotypes was investigated in persons who were ill with acute gastroenteritis associated with the consumption of oysters. Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs was applied to nucleic acid extracted from faecal specimens collected from symptomatic cases. Using primers that amplified contiguous sequences in the ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, three different GII and two GI NV genotypes were detected and the strains were characterised by DNA sequencing. Using this approach a recombinant NV genotype, rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several symptomatic cases from the outbreak, was detected and characterised. No other gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 40/41 were detected by RT-PCR and PCR.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ostreidae
/
Mariscos
/
Infecciones por Caliciviridae
/
Norovirus
/
Gastroenteritis
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Int J Food Microbiol
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido