Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.
Immunol Lett
; 100(2): 120-9, 2005 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-16154492
ABSTRACT
We have reported earlier that purified preparations of sheep fetal hemoglobin, but not adult hemoglobin, in concert with non-stimulatory doses of lipopolysaccharide (LPS) (lipid A), act cooperatively to regulate in vitro production of a number of cytokines, including TNFalpha, TGFbeta and IL-6 from murine and human leukocytes. Following in vivo treatment of mice with the same combination of hemoglobin and LPS, harvested spleen or peritoneal cells showed a similar augmented capacity to release these cytokines into culture supernatants. We report below that genetically cloned gamma-chain of human or sheep fetal hemoglobin, but not cloned alpha- or beta-chains, can produce this cooperative effect, as indeed can HPLC purified, heme-free, gamma-chains derived from cord blood fetal hemoglobin, and that purified haptoglobin completely abolishes the cooperative interaction.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Hemoglobina Fetal
/
Globinas
/
Linfocitos
/
Lipopolisacáridos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Immunol Lett
Año:
2005
Tipo del documento:
Article
País de afiliación:
Canadá