Radiation responses of perfused tracheal tissue.
Radiat Res
; 164(4 Pt 2): 487-92, 2005 Oct.
Article
en En
| MEDLINE
| ID: mdl-16187754
ABSTRACT
We are using a novel perfusion system to examine the effects of radiation on a model respiratory tissue. Tracheas taken from young adult male Fischer 344 rats are embedded in a growth factor-enriched agarose matrix that is mounted in a special apparatus designed to allow growth medium to periodically wash the epithelial surface of the lumen. A comparison of the microarray expression profiles of freshly harvested tracheas and tracheas maintained in perfusion culture for 24 h shows no significant difference except for an increase in expression of a few metabolism- and surfactant-related genes. Perfusion culture samples exposed to 4 Gy of X rays show a lower than expected increase in expression for some cell cycle- and repair-related genes.
Palabras clave
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tráquea
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Radiat Res
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos