Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid.
J Agric Food Chem
; 53(20): 7673-80, 2005 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-16190615
ABSTRACT
The immunomodulatory activities of four sialic acid-containing milk proteins (kappa-casein, glycomacropeptide, lactoferrin, and proteose peptone-3 component) were determined, and the role of sialic acid was evaluated. Two in vitro models were used murine splenocyte proliferation, where the effect on LPS-, Con A-, and PHA-stimulated proliferation was studied, and cytokine production in LPS-stimulated murine dendritic cells (DC). All four proteins inhibited LPS-induced splenocyte proliferation, though to different degrees, and independently of sialic acid. kappa-Casein strongly inhibited PHA-induced proliferation and had a weak inhibitory effect on Con A-induced proliferation, whereas lactoferrin stimulated Con A-induced proliferation. kappa-Casein, glycomacropeptide, and lactoferrin differentially affected cytokine production by DC kappa-casein significantly inhibited production of TNF-alpha, IL-10, -12, -6, and -1beta, independent of sialic acid, whereas less-marked effects of glycomacropeptide and lactoferrin were seen. These findings thus point to important immunosuppressive effects of some milk proteins and indicate that they may function via different mechanisms.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ácido N-Acetilneuramínico
/
Inmunidad
/
Proteínas de la Leche
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Agric Food Chem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Dinamarca