Dual-probe assay for detection of lamivudine-resistance hepatitis B virus by real-time PCR.
J Virol Methods
; 132(1-2): 25-31, 2006 Mar.
Article
en En
| MEDLINE
| ID: mdl-16198006
ABSTRACT
A rapid and accurate minor groove binder (MGB) real-time PCR is described for detection lamivudine resistance mutations in hepatitis B virus. The real-time PCR was compared with direct Sanger sequencing in 53 clinical patients samples, the results of the real-time PCR correlate with the nucleotide sequence and the assay has the advantage of detecting a mixture of quasi-species with higher sensitivity than sequencing. As a simple, easy, rapid, accurate and high throughout method, MGB real-time PCR assay should be useful for detecting lamivudine resistance variants during lamivudine therapy in clinical settings.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Viral
/
Virus de la Hepatitis B
/
Reacción en Cadena de la Polimerasa
/
Lamivudine
/
Farmacorresistencia Viral
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
2006
Tipo del documento:
Article
País de afiliación:
China