Liquid-based hybridization assay with real-time detection in miniaturized array platforms.
Biomol Eng
; 23(1): 35-40, 2006 Mar.
Article
en En
| MEDLINE
| ID: mdl-16298548
ABSTRACT
An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Dermatoglifia del ADN
/
Hibridación Fluorescente in Situ
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Transferencia Resonante de Energía de Fluorescencia
/
Técnicas Analíticas Microfluídicas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Biomol Eng
Asunto de la revista:
BIOLOGIA MOLECULAR
/
ENGENHARIA BIOMEDICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Alemania