Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora.
Environ Microbiol
; 7(12): 1996-2010, 2005 Dec.
Article
en En
| MEDLINE
| ID: mdl-16309396
ABSTRACT
A metagenome expression library of bulk DNA extracted from the rumen content of a dairy cow was established in a phage lambda vector and activity-based screening employed to explore the functional diversity of the microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-beta-1,4-glucanases and one cyclodextrinase) were identified in the library and characterized. Sequence analysis of the retrieved enzymes revealed that eight (36%) were entirely new and formed deep-branched phylogenetic lineages with no close relatives among known ester- and glycosyl-hydrolases. Bioinformatic analyses of the hydrolase gene sequences, and the sequences and contexts of neighbouring genes, suggested tentative phylogenetic assignments of the rumen organisms producing the retrieved enzymes. The phylogenetic novelty of the hydrolases suggests that some of them may have potential for new applications in biocatalysis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Rumen
/
Proteínas Bacterianas
/
Proteínas Fúngicas
/
Hidrolasas
Límite:
Animals
País/Región como asunto:
Oceania
Idioma:
En
Revista:
Environ Microbiol
Asunto de la revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania