DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics.
Bull Entomol Res
; 95(6): 605-13, 2005 Dec.
Article
en En
| MEDLINE
| ID: mdl-16336708
ABSTRACT
The two most widespread biotypes of Bemisia tabaci (Gennadius) in southern Europe and the Middle East are referred to as the B and Q-type, which are morphologically indistinguishable. In this study various DNA markers have been developed, applied and compared for studying genetic diversity and distribution of the two biotypes. For developing sequence characterized amplified regions (SCAR) and cleaved amplified polymorphic sequences (CAPS) techniques, single random amplified polymorphic DNA (RAPD) fragments of B and Q biotypes, respectively, were used. The CAPS were investigated on the basis of nuclear sodium channel and the mitochondrial cytochrome oxidase I genes (mtCOI) sequences. In general, complete agreement was found between the different markers used. Analysis of field samples collected in Israel for several years, using these markers, indicated that the percentage of the Q biotype tends to increase in field populations as time progresses. This may be attributed to the resistance of the Q biotype to neonicotinoids and pyriproxyfen and the susceptibility of the B biotype to these insecticides.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hemípteros
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Bull Entomol Res
Año:
2005
Tipo del documento:
Article
País de afiliación:
Israel