Monoclonal antibody C242-Pseudomonas exotoxin A. A specific and potent immunotoxin with antitumor activity on a human colon cancer xenograft in nude mice.
J Clin Invest
; 90(2): 405-11, 1992 Aug.
Article
en En
| MEDLINE
| ID: mdl-1644913
ABSTRACT
Two immunotoxins were constructed by chemically coupling the monoclonal antibody C242 to Pseudomonas exotoxin A (PE) or a modified form, NlysPE40, that lacks the cell binding domain of PE. Monoclonal antibody C242 recognizes a specific sialylated carbohydrate epitope on a high molecular weight membrane glycoprotein present on cells of human colon, pancreatic, and cervical cancers. C242-PE and C242-NlysPE40 were very cytotoxic for cells expressing this antigen with 50% inhibition of protein synthesis occurring on Colo205 cells at 0.2 ng/ml (0.9 pM) for C242-PE and 6.0 ng/ml (31 pM) for C242-NlysPE40. The two immunotoxins also exhibited a strong antitumor effect on a human colon cancer xenograft grown in nude mice. The specificity and potency of these two C242 immunotoxins warrant their further development for the treatment of cancer.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Toxinas Bacterianas
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Antígenos de Carbohidratos Asociados a Tumores
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Inmunotoxinas
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ADP Ribosa Transferasas
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Neoplasias del Colon
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Factores de Virulencia
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Exotoxinas
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Anticuerpos Monoclonales
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Anticuerpos Antineoplásicos
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Clin Invest
Año:
1992
Tipo del documento:
Article