Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches.
Ann N Y Acad Sci
; 1063: 35-46, 2005 Dec.
Article
en En
| MEDLINE
| ID: mdl-16481488
ABSTRACT
The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high-throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Rickettsia prowazekii
/
Genoma Bacteriano
/
Proteómica
Límite:
Humans
Idioma:
En
Revista:
Ann N Y Acad Sci
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos