Polyinosinic-polycytidylic acid induces the expression of GRO-alpha in BEAS-2B cells.
Inflammation
; 29(1): 17-21, 2005 Feb.
Article
en En
| MEDLINE
| ID: mdl-16502342
ABSTRACT
Growth-related oncogene protein-alpha (GRO-alpha)/CXCLl is a chemokine that activates neutrophils and plays an important role in inflammatory reactions. Polyinosinic-polycytidylic acid (poly IC) is a synthetic double-stranded RNA (dsRNA), which is a ligand for Toll-like receptor-3. Poly IC mimics viral infection when applied to cells and induces inflammatory and immune responses. In the present study, we found the induction of GRO-alpha in BEAS-2B bronchial epithelial cells treated with poly IC. Pretreatment of cells with 2-aminopurine, an inhibitor for dsRNA-dependent protein kinase (PKR), inhibited the expression of GRO-alpha-induced by poly IC. Overexpression of interferon-regulatory factor-3 (IRF-3) or retinoic-acid inducible gene-I (RIG-I) enhanced the induction of GRO-alpha by poly IC. PKR, IRF-3, and RIG-I may be involved in the poly IC-induced expression of GRO-alpha in BEAS-2B cells. Airway viral infection may elicit GRO-alpha expression in the bronchial epithelium, which may be implicated in inflammatory and immune reactions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bronquios
/
Inductores de Interferón
/
Poli I-C
/
Quimiocinas CXC
/
Péptidos y Proteínas de Señalización Intercelular
/
Células Epiteliales
Límite:
Humans
Idioma:
En
Revista:
Inflammation
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón