Human polymorphonuclear leukocytes produce and express functional tissue factor upon stimulation.
J Thromb Haemost
; 4(6): 1323-30, 2006 Jun.
Article
en En
| MEDLINE
| ID: mdl-16706978
ABSTRACT
BACKGROUND:
Blood-borne tissue factor (TF) plays a crucial role in thrombogenesis.AIM:
To study whether polymorphonuclear leukocytes (PMN) are a source of TF. METHODS ANDRESULTS:
Human PMN were carefully separated from other blood cells and stimulated for 3 min with purified P-selectin or the chemotactic peptide formyl-MetLeuPhe (fMLP) they expressed both TF procoagulant activity, as identified by specific TF MoAb and inactivated factor VIIa blockade; and TFAg (four to six times), as shown by flow-cytometry and immunocytochemistry. About 40% of permeabilized PMN, both resting and stimulated, contained TFAg, indicating that stimulation only modifies the location of TFAg within PMN. By real time-polymerase chain reaction (RT-PCR), a very low amount of TF mRNA was detectable in resting PMN, but a 3- to 5-fold increase was observed after 1-h stimulation with P-selectin or fMLP, respectively.CONCLUSIONS:
These findings suggest that TF is not constitutively expressed in peripheral PMN, but can be up-regulated and produced upon stimulation and specific gene transcription, as for instance during contact with activated platelets or endothelium. The stored TF is rapidly expressed in vitro as a functional molecule on the surface of activated PMN. The availability of PMN TF supports the relevance of inflammatory cells and their interaction with platelets for fibrin deposition and thrombus formation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Coagulación Sanguínea
/
Tromboplastina
/
Regulación de la Expresión Génica
/
Neutrófilos
Límite:
Humans
Idioma:
En
Revista:
J Thromb Haemost
Asunto de la revista:
HEMATOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Italia