The sea urchin embryo as a model for studying efflux transporters: roles and energy cost.
Mar Environ Res
; 62 Suppl: S1-4, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16740304
ABSTRACT
We describe the use of the sea urchin as a model for studying efflux transporters and estimating energy cost for the cytotoxin protective system provided by these transporters. The unfertilized egg has low transport activity, which increases to a new steady state shortly after fertilization. Activity results from p-glycoprotein (p-gp) and MRP type transporters which protect the embryo from cytotoxic drugs that can disrupt cell division or induce apoptosis. The energy cost is estimated from a novel use of calcein-AM as a substrate; keeping 0.25 microM substrate levels out of the cell utilizes only 0.023% of steady state respiration.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Transporte de Membrana
/
Embrión no Mamífero
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Metabolismo Energético
/
Fluoresceínas
Tipo de estudio:
Health_economic_evaluation
Límite:
Animals
Idioma:
En
Revista:
Mar Environ Res
Asunto de la revista:
BIOLOGIA
/
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos