Leishmania donovani infection of human myeloid dendritic cells leads to a Th1 response in CD4+ T cells from healthy donors and patients with kala-azar.
J Infect Dis
; 194(3): 294-301, 2006 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-16826476
ABSTRACT
The role played by dendritic cells (DCs) in Leishmania donovani infection is poorly understood. Here, we report that L. donovani amastigotes efficiently infect human peripheral-blood monocyte-derived DCs. Opsonization with normal human serum enhanced the infectivity of amastigotes and promastigotes only marginally. Surface attachment versus internalization was distinguished by incubation of DCs with live, fluorescein isothiocyanate-labeled parasites, followed by quenching with crystal violet. Infection with amastigotes was accompanied by DC maturation, as was evident from the up-regulation of maturation-associated cell-surface markers, the nuclear translocation of RelB, and the release of cytokines. Amastigote-primed DCs produced inflammatory cytokines in response to subsequent treatment with interferon- gamma or anti-CD40 monoclonal antibody. When cocultured, amastigote-infected DCs induced T helper cell type 1 (Th1) responses both in naive allogeneic CD4(+) T cells and in autologous CD4(+) T cells from patients with kala-azar and up-regulated the expression of T-bet. Our data reveal that infection with L. donovani amastigotes induces a Th1 cytokine milieu in both DCs and T cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Leishmania donovani
/
Células Dendríticas
/
Linfocitos T CD4-Positivos
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Células TH1
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Leishmaniasis Visceral
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Infect Dis
Año:
2006
Tipo del documento:
Article
País de afiliación:
India