Identification of alternative amino acid substitutions in drug-resistant variants of the HIV-1 reverse transcriptase.
AIDS
; 20(11): 1515-20, 2006 Jul 13.
Article
en En
| MEDLINE
| ID: mdl-16847406
ABSTRACT
OBJECTIVE/DESIGN:
To identify new drug-resistance-associated mutations in the HIV-1 reverse transcriptase (RT) protein, we screened the RT sequence database of our hospital for alternative amino acid substitutions at known RT drug-resistance positions.METHOD:
The genotypic database used for this analysis contained 1322 RT sequences from 1015 patients. We analysed this RT database with a focus on alternative mutations at RT positions known to be involved in drug resistance. The patterns of drug resistance associated with these alternative mutations were investigated in a separate database containing genotype and drug-susceptibility results.RESULTS:
We identified multiple alternative resistance-associated mutations at amino acid positions 44, 62, 67, 69, 70, 74, 75, 103, 181, 190, 210, and 219 in RT. Phenotypic analysis indicated that drug-resistance properties of the alternative Y181V and L74I mutants are similar, but not identical, to that of the well-known Y181C and L74V mutations.CONCLUSION:
This initial survey indicates that many resistance-associated phenomena can be distilled from existing data. These findings endorse a more extensive analysis by computerized methods.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
VIH-1
/
Inhibidores de la Transcriptasa Inversa
/
Fármacos Anti-VIH
/
Farmacorresistencia Viral
/
Transcriptasa Inversa del VIH
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
AIDS
Asunto de la revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Año:
2006
Tipo del documento:
Article
País de afiliación:
Países Bajos