Circulating blood volume determination using electronic spin resonance spectroscopy.
Appl Spectrosc
; 60(9): 1078-84, 2006 Sep.
Article
en En
| MEDLINE
| ID: mdl-17002834
ABSTRACT
There have been numerous methods proposed to measure the circulating blood volume (CBV). Nevertheless, none of them have been massively and routinely accepted in clinical diagnosis. This study describes a simple and rapid method, on a rabbit model, using the dilution of autologous red cells labeled with a nitroxide radical (Iodoacetamide-TEMPO), which can be detected by electronic spin resonance (ESR) spectroscopy. Blood samples were withdrawn and re-injected using the ears' marginal veins. The average CBV measured by the new method/body weight (CBV(IAT)/BW) was 59 +/- 7 mL/kg (n = 33). Simultaneously, blood volume determinations using the nitroxide radical and (51)Cr (CBV(Cr)) were performed. In the plot of the difference between the methods (CBV(IAT) - CBV(Cr)) against the average (CBV(IAT) + CBV(Cr))/2, the mean of the bias was -1.1 +/- 6.9 mL and the limits of agreement (mean difference +/-2 SD) were -14.9 and 12.7 mL. Lin's concordance correlation coefficient p(c) = 0.988. Thus, both methods are in close agreement. The development of a new method that allows a correct estimation of the CBV without using radioactivity, avoiding blood manipulation, and decreasing the possibility of blood contamination with similar accuracy and precision of that of the "gold standard method" is an innovative proposal.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Circulación Sanguínea
/
Determinación del Volumen Sanguíneo
Límite:
Animals
Idioma:
En
Revista:
Appl Spectrosc
Año:
2006
Tipo del documento:
Article
País de afiliación:
Argentina