Differential regulation of rat T-kininogen by tumor necrosis factor and interleukin-6.
Mol Endocrinol
; 4(4): 543-50, 1990 Apr.
Article
en En
| MEDLINE
| ID: mdl-1704100
ABSTRACT
Rat T-kininogen (T-KG), a cysteine protease inhibitor, is an acute phase reactant which is induced to high levels in response to inflammation. Both hormones and cytokines participate in this regulation. To investigate the cis-acting elements responsible for the induction of gene expression, various 5'-fragments of the rat T-KG gene were fused to a chloramphenicol acetyltransferase marker gene. These constructs were transfected into a rat hepatoma cell line which was then treated with tumor necrosis factor or interleukin-6 or both cytokines. Expression of the chloramphenicol acetyltransferase gene was induced with interleukin-6 treatment, but suppressed by tumor necrosis factor. The 5'-region of the T-KG gene responsible for conferring both of these effects was localized between nucleotides -404 to -210 upstream of the transcription start site. Fragments containing this region were found to be effective in either orientation, and could also regulate a heterologous promoter.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Fase Aguda
/
Regulación de la Expresión Génica
/
Quininógenos
/
Interleucina-6
/
Factor de Necrosis Tumoral alfa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Endocrinol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
ENDOCRINOLOGIA
Año:
1990
Tipo del documento:
Article