[Study on fingerprints of astragalosides injection by HPLC-ELSD].
Zhongguo Zhong Yao Za Zhi
; 31(10): 807-9, 2006 May.
Article
en Zh
| MEDLINE
| ID: mdl-17048660
ABSTRACT
OBJECTIVE:
To develop an HPLC method for fingerprint determination of the astragalosides injection.METHOD:
Analyses were carried out at 25 degrees C on a Zorbax SB-C18 column (4.6 mm x 250 mm,5 microm). Mobile phase A was water, and B was acetonitrile. The analysis followed a linear gradient program. Initial conditions were 15% B; 0 to approximately 65 min, changed to 60% B. The flow rate was 0.8 mL x min(-1). The drift tube temparature of the ELSD was 105 degrees C, and gas flowrate was 2.7 L x min(-1).RESULT:
The RSD of relative retention time of the common peaks in the plant material, the extract and the injection fingerprints was less than 0.1%, and there was good similarity among 10 batches of samples.CONCLUSION:
The method was reliable and simple which could be used for quality control of the injection.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plantas Medicinales
/
Saponinas
/
Triterpenos
/
Medicamentos Herbarios Chinos
/
Astragalus propinquus
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2006
Tipo del documento:
Article
País de afiliación:
China