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Micro-array analysis of resistance for gemcitabine results in increased expression of ribonucleotide reductase subunits.
Smid, K; Bergman, A M; Eijk, P P; Veerman, G; van Haperen, V W T Ruiz; van den Ijssel, P; Ylstra, B; Peters, G J.
Afiliación
  • Smid K; Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands.
Nucleosides Nucleotides Nucleic Acids ; 25(9-11): 1001-7, 2006.
Article en En | MEDLINE | ID: mdl-17065054
ABSTRACT
To study in detail the relation between gene expression and resistance against gemcitabine, a cell line was isolated from a tumor for which gemcitabine resistance was induced in vivo. Similar to the in vivo tumor, resistance in this cell line, C 26-G, was not related to deficiency of deoxycytidine kinase (dCK). Micro-array analysis showed increased expression of ribonucleotide reductase (RR) subunits M1 and M2 as confirmed by real time PCR analysis (28- and 2.7-fold, respectively). In cell culture, moderate cross-resistance (about 2-fold) was observed to 1-ss-D-arabinofuranosylcytosine (ara-C), 2-chloro-2'deoxyadenosine (CdA), LY231514 (ALIMTA), and cisplatin (CDDP), and pronounced cross-resistance (>23-fold) to 2',2'-difluorodeoxyuridine (dFdU) and 2',2'-difluorodeoxyguanosine (dFdG). Culture in the absence of gemcitabine reduced resistance as well as RRM1 RNA expression, demonstrating a direct relationship of RRM1 RNA expression with acquired resistance to gemcitabine.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ribonucleótido Reductasas / Regulación Neoplásica de la Expresión Génica / Resistencia a Antineoplásicos / Análisis de Secuencia por Matrices de Oligonucleótidos / Desoxicitidina Límite: Humans Idioma: En Revista: Nucleosides Nucleotides Nucleic Acids Asunto de la revista: BIOQUIMICA Año: 2006 Tipo del documento: Article País de afiliación: Países Bajos
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ribonucleótido Reductasas / Regulación Neoplásica de la Expresión Génica / Resistencia a Antineoplásicos / Análisis de Secuencia por Matrices de Oligonucleótidos / Desoxicitidina Límite: Humans Idioma: En Revista: Nucleosides Nucleotides Nucleic Acids Asunto de la revista: BIOQUIMICA Año: 2006 Tipo del documento: Article País de afiliación: Países Bajos