Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells.
J Cell Biol
; 176(2): 173-82, 2007 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-17227892
ABSTRACT
How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ciclo Celular
/
Centriolos
/
Centrosoma
/
Células Epiteliales
Límite:
Humans
Idioma:
En
Revista:
J Cell Biol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos