Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion.
J Leukoc Biol
; 81(5): 1205-12, 2007 May.
Article
en En
| MEDLINE
| ID: mdl-17307863
ABSTRACT
Similar to the regulation of vasodilation, the balance between NO and superoxide (O2-) regulates expansion of activated T cells in mice. Reduction of suppressive NO levels by O2- is essential for T cell expansion and development of autoimmunity. In mice primed with heat-killed Mycobacterium, a splenocyte population positive for Gr-1 (Ly-6G/C) is the exclusive source of both immunoregulatory free radicals. Distinct Gr-1+ cell subpopulations were separated according to Ly-6G expression. In culture with activated T cells, predominantly monocytic Ly-6G- Gr-1+ cells produced T cell-inhibitory NO but no O2-. However, mostly granulocytic Ly-6G+ cells produced O2- simultaneously but had no measurable effect on proliferation. Recombination of the two purified Gr-1+ subpopulations restored controlled regulation of T cell proliferation through NO and O2- interaction. Coculture of p47phox-/- and inducible NO synthase-/- Gr-1+ cells confirmed this intercellular interaction. These data suggest that bacterial products induce development of distinct Gr-1+ myeloid lineages, which upon stimulation by activated T cells, interact via their respective free radical products to modulate T cell expansion.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Linfocitos T
/
Linaje de la Célula
/
Receptores de Quimiocina
/
Células Mieloides
/
Mycobacterium tuberculosis
Límite:
Animals
Idioma:
En
Revista:
J Leukoc Biol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos