Development of a time-resolved method for photodissociation mechanistic study of protonated peptides: use of a voltage-floated cell in a tandem time-of-flight mass spectrometer.
J Am Soc Mass Spectrom
; 18(10): 1729-39, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17702598
ABSTRACT
Photodissociation at 266 nm of some protonated peptides was investigated using a tandem-TOF spectrometer equipped with a cell near its first time focal point where the laser was irradiated. When a high voltage was applied to the cell, each product ion peak split into several components with different flight times. One of these was due to in-cell direct formation of the product ion and another due to post-cell formation. Those in between were due to consecutive dissociations, the first steps of which occurred inside the cell and the second steps outside the cell. A method based on flight time calculation was developed to analyze these components and to identify the intermediate ion for each consecutive component. The technique allows time-resolved photodissociation mechanistic studies on a 100-ns timescale.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Fotoquímica
/
Mapeo Peptídico
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2007
Tipo del documento:
Article