Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method.
J Clin Virol
; 40(2): 129-34, 2007 Oct.
Article
en En
| MEDLINE
| ID: mdl-17728179
ABSTRACT
We report the use of a prototype Invader Plus method (Third Wave Technologies, Inc., Madison, WI) for the qualitative detection of varicella-zoster virus (VZV) and differentiation of wild-type and Oka vaccine VZV. The analytical sensitivity of the VZV Invader Plus reagents is at 10 copies per reaction. A total of 174 skin and mucous swab specimens were used to validate the assay's performance. The sensitivity and specificity were 98.3% and 98.1%, respectively, in comparison to a PCR-EIA assay. A perfect 100% agreement was obtained when VZV wild-type and vaccine differentiation was performed on 54 VZV-positive swab specimens against an allele-specific FRET real-time assay. The Invader Plus method provides another reliable tool for qualitative detection of VZV and differentiation of wild-type and vaccine virus.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Varicela
/
Herpesvirus Humano 3
/
Vacuna contra la Varicela
/
Herpes Zóster
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
J Clin Virol
Asunto de la revista:
VIROLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos