Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family.
FEBS Lett
; 581(24): 4657-62, 2007 Oct 02.
Article
en En
| MEDLINE
| ID: mdl-17826771
ABSTRACT
An acetylxylan esterase (R.44), belonging to the carbohydrate esterase family 6 (CE6), retrieved from bovine rumen metagenome was analyzed. Molecular modelling and site-directed mutagenesis indicated that the enzyme possesses a catalytic triad formed by Ser(14), His(231) and Glu(152). The catalytic Ser and His have been identified in highly conserved sequences GQSX and DXXH in the CE6 family, respectively, and the active-site glutamate was part of a highly conserved sequence HQGE. This motif is situated near to the so-called Block III in the CE6 family and its role in catalysis has not been identified so far.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Esterasas
/
Metabolismo de los Hidratos de Carbono
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
España