Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture.
Biomaterials
; 28(36): 5518-25, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17881048
ABSTRACT
We describe a method for creating alginate hydrogels with adjustable degradation rates that can be used as scaffolds for stem cells. Alginate hydrogels have been widely tested as three-dimensional constructs for cell culture, cell carriers for implantation, and in tissue regeneration applications; however, alginate hydrogel implants can take months to disappear from implantation sites because mammals do not produce endogenous alginases. By incorporating poly(lactide-co-glycolide) (PLGA) microspheres loaded with alginate lyase into alginate hydrogels, we demonstrate that alginate hydrogels can be enzymatically degraded in a controlled and tunable fashion. We demonstrate that neural progenitor cells (NPCs) can be cultured and expanded in vitro in this degradable alginate hydrogel system. Moreover, we observe a significant increase in the expansion rate of NPCs cultured in degrading alginate hydrogels versus NPCs cultured in standard, i.e. non-degrading, alginate hydrogels. Degradable alginate hydrogels encapsulating stem cells may be widely applied to develop novel therapies for tissue regeneration.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Poliglactina 910
/
Células Madre
/
Técnicas de Cultivo de Célula
/
Hidrogeles
/
Alginatos
Límite:
Animals
Idioma:
En
Revista:
Biomaterials
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos