Nisin-controlled extracellular production of interleukin-2 in Lactococcus lactis strains, without the requirement for a signal peptide sequence.

Fernández, Antonio; Rodríguez, Juan M; Bongaerts, Roy J; Gasson, Michael J; Horn, Nikki

Fernández, Antonio; Rodríguez, Juan M; Bongaerts, Roy J; Gasson, Michael J; Horn, Nikki.

Afiliación

Fernández A; Commensals & Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. antonio.dumont@bbsrc.ac.uk

Appl Environ Microbiol ; 73(23): 7781-4, 2007 Dec.

Article en En | MEDLINE | ID: mdl-17905884

ABSTRACT

ABSTRACT

Secretion of the cytokine interleukin-2 (IL-2) was investigated in Lactococcus lactis using the secretory machinery of the bacteriocin lactococcin A. Surprisingly, the lcnCD transport genes were not essential for mouse IL-2 secretion. Furthermore, expression of a mature mouse IL-2 gene resulted in interleukin secretion without the requirement for a leader sequence.

Asunto(s)

Interleucina-2/metabolismo; Lactococcus lactis/metabolismo; Nisina/genética; Señales de Clasificación de Proteína/genética; Animales; Bacteriocinas/genética; Bacteriocinas/metabolismo; Western Blotting; L-Lactato Deshidrogenasa/genética; L-Lactato Deshidrogenasa/metabolismo; Lactococcus lactis/genética; Ratones; Regiones Promotoras Genéticas/genética

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Señales de Clasificación de Proteína / Interleucina-2 / Lactococcus lactis / Nisina Límite: Animals Idioma: En Revista: Appl Environ Microbiol Año: 2007 Tipo del documento: Article País de afiliación: Reino Unido

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