Association of BAF53 with mitotic chromosomes.
Mol Cells
; 24(2): 288-93, 2007 Oct 31.
Article
en En
| MEDLINE
| ID: mdl-17978584
ABSTRACT
The conversion of mitotic chromosome into interphase chromatin consists of at least two separate processes, the decondensation of the mitotic chromosome and the formation of the higher-order structure of interphase chromatin. Previously, we showed that depletion of BAF53 led to the expansion of chromosome territories and decompaction of the chromatin, suggesting that BAF53 plays an essential role in the formation of higher-order chromatin structure. We report here that BAF53 is associated with mitotic chromosomes during mitosis. Immunostaining with two different anti-BAF53 antibodies gave strong signals around the DNA of mitotic preparations of NIH3T3 cells and mouse embryo fibroblasts (MEFs). The immunofluorescent signals were located on the surface of mitotic chromosomes prepared by metaphase spread. BAF53 was also found in the mitotic chromosome fraction of sucrose gradients. Association of BAF53 with mitotic chromosomes would allow its rapid activation on the chromatin upon exit from mitosis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Cromosómicas no Histona
/
Actinas
/
Cromosomas de los Mamíferos
/
Proteínas de Unión al ADN
/
Mitosis
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Cells
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2007
Tipo del documento:
Article