[Protective effect of amlodipine on the cytotoxicity induced by contrast media in human kidney cells].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 32(5): 806-11, 2007 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-18007075
ABSTRACT
OBJECTIVE:
To explore the protective effect of amlodipine on the cytotoxicity induced by contrast media (meglumine diatrizoate) in human kidney cells (HKC).METHODS:
An HKC line was used. The experiment was divided into 4 groups a model group (diatrizoate 111g/L), a prevention group (diatrizoate 111g/L+amlodipine 10(-5)mol/L), an amlodipine control group (amlodipine 10(-5)mol/L), and a culture medium control group (simple none blood serum DMEM-F12 medium). Cytotoxicity induced by meglumine diatrizoate was analysed by methyl thiazolyl tetrazolium (MTT) test, lactate dehydrogenase (LDH) assay, Hochest33258 fluorescence stained cytospins, and flow cytometric DNA analysis. The protein expression of Bax was determined by Western blot, and caspase-3 activity was examined by fluorometric method.RESULTS:
In the prevention group, the cell viability increased significantly (P<0.05), LDH levels decreased (P<0.05), and the apoptosis was lower than that of the model group (P<0.05) .Bax protein expression and caspase 3 activity decreased (P<0.05).CONCLUSION:
Amlodipine can inhibit the HKC apoptosis and protect the renal tubule cell from injury induced by meglumine diatrizoate.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Amlodipino
/
Medios de Contraste
/
Células Epiteliales
/
Túbulos Renales
Límite:
Humans
Idioma:
Zh
Revista:
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China