Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins.
J Chromatogr A
; 1176(1-2): 192-205, 2007 Dec 28.
Article
en En
| MEDLINE
| ID: mdl-18036598
ABSTRACT
Proteins in bile may have important physiological functions and serve as disease biomarkers. Here, the protein composition of human gallbladder bile was analyzed using a recently described chromatography-like technology capable to enhance the signal of low-abundance species. First, proteins present in bile fluid were treated with immobilized peptide ligand libraries to concentrate dilute and very dilute species while concomitantly diluting the high-abundance proteins. The analysis of resulting protein mixture was then performed using LC-MS/MS after having classically separated proteins by a mini preparative gel electrophoresis. Overall 222 gene products were found; 143 of them were not reported before in proteomics studies. Ligand libraries by themselves contributed to find 81 new gene products distributed throughout different categories. The described chromatographic approach provides a significant contribution to the bile protein repertoire and opens new perspectives for the discovery of markers for specific biliary tract diseases.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oligopéptidos
/
Bilis
/
Proteínas
/
Biblioteca de Péptidos
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia