Temporal evolution of soluble Fas and Fas ligand in patients with orthotopic liver transplantation.
Cytokine
; 41(3): 240-3, 2008 Mar.
Article
en En
| MEDLINE
| ID: mdl-18267366
ABSTRACT
AIM:
The aim of this study was to analyze the expression levels of plasma soluble Fas (sFas) and soluble Fas ligand (sFasL) in patients with orthotopic liver transplantation (OLT) procedures routinely performed without venovenous bypass.METHODS:
The sFas and sFasL were analyzed in the blood of 20 consecutive patients who underwent transplantation. Blood samples were drawn from the radial artery at serial time points before, during, and after surgery. Plasma levels of sFas and sFasL were detected by Enzyme Linked-Immuno-Sorbent Assay. Plasma aspartate transaminase (AST) and alanine transaminase (ALT) were assayed by routine clinical chemistry testing.RESULTS:
Marked elevation of plasma AST and ALT were detected at the reperfusion and postoperation time points (P<0.001), with a peak on the first postoperative day. The mean plasma concentration of sFas and sFasL remained unchanged from preoperative to anhepatic phase (T1 to T3) (P> or =0.268). The sFas and sFasL concentrations were significantly higher at 15 and 60 min after reperfusion compared to the preoperative value (P< or =0.048). Postoperatively, sFas and sFasL concentration were decreased to preoperative levels on the first postoperative day (P> or =0.127).CONCLUSION:
The sFas and sFasL seem to be involved in reperfusion injury during OLT. The understanding of Fas may provide new insights into the mechanisms of ischemia/reperfusion injury during OLT.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Daño por Reperfusión
/
Trasplante de Hígado
/
Receptor fas
/
Proteína Ligando Fas
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Cytokine
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2008
Tipo del documento:
Article