Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry.
Anal Bioanal Chem
; 390(7): 1843-52, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-18274736
ABSTRACT
The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC-electrospray-ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This "top-down" approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factor I del Crecimiento Similar a la Insulina
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Animals
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia