Time-resolved photodissociation of singly protonated peptides with an arginine at the N-terminus: a statistical interpretation.
J Am Soc Mass Spectrom
; 19(5): 645-55, 2008 May.
Article
en En
| MEDLINE
| ID: mdl-18356076
ABSTRACT
Time-evolution of product ion signals in ultraviolet photodissociation (UV-PD) of singly protonated peptides with an arginine at the N-terminus was investigated by using a tandem time-of-flight mass spectrometer equipped with a cell floated at high voltage. Observation of different time-evolution patterns for different product ion types--an apparently nonstatistical behavior--could be explained within the statistical framework by invoking consecutive formation of some product ions and broad internal energy distributions for precursor ions. a(n) + 1 and b(n) ions were taken as the primary product ions from this type of peptide ions. Spectral characteristics in post-source decay, UV-PD, and collisionally activated dissociation at low and high kinetic energies could be explained via rough statistical calculation of rate constants. Specifically, the striking characteristics in high-energy CAD and UV-PD--dominance of a(n) and d(n) formed via a(n) + 1--were not due to the peculiarity of the excitation processes themselves, but due to quenching of the b(n) channels caused by the presence of arginine.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
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Fotoquímica
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Arginina
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Algoritmos
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Mapeo Peptídico
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Interpretación Estadística de Datos
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2008
Tipo del documento:
Article