[Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(8): 1327-30, 2008 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-18753051
ABSTRACT
OBJECTIVE:
To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.METHODS:
Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis. RESULTS ANDCONCLUSION:
The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Calicreínas
/
Cadherinas
/
Vectores Genéticos
/
Glutatión Peroxidasa
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2008
Tipo del documento:
Article
País de afiliación:
China