A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants.
Nucleic Acids Res
; 36(20): e130, 2008 Nov.
Article
en En
| MEDLINE
| ID: mdl-18794177
ABSTRACT
Studies of gene fusions in solid tumors are not as extensive as in hematological malignancies due to several technical and analytical problems associated with tumor heterogeneity. Nevertheless, there is a growing interest in the role of fusion genes in common epithelial tumors after the discovery of recurrent TMPRSS2ETS fusions in prostate cancer. Among all of the reported fusion partners in the ETS gene family, TMPRSS2ERG is the most prevalent one. Here, we present a simple and sensitive microarray-based assay that is able to simultaneously determine multiple fusion variants with a single RT-PCR in impure RNA specimens. The assay detected TMPRSS2ERG fusion transcripts with a detection sensitivity of <10 cells in the presence of more than 3000 times excess normal RNA, and in primary prostate tumors having no >1% of cancer cells. The ability to detect multiple transcript variants in a single assay is critically dependent on both the primer and probe designs. The assay should facilitate clinical and basic studies for fusion gene screening in clinical specimens, as it can be readily adapted to include multiple gene loci.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Proteínas de Fusión Oncogénica
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Análisis de Secuencia por Matrices de Oligonucleótidos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos