Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches.
Anal Chim Acta
; 629(1-2): 158-64, 2008 Nov 23.
Article
en En
| MEDLINE
| ID: mdl-18940332
ABSTRACT
Preeclampsia (PE) is a complex and serious condition of pregnancy. Trophoblasts in human placenta can be separated and collected by laser capture micro-dissection (LCM). Protein in trophoblasts have been extracted and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), finally 962 unique proteins are identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). Comparison of differential expressed proteins in normal and those in PE are investigated. Two-dimensional electrophoresis (2DE) and MS were used to identify differential expressed proteins. 13 differential expressed proteins include signal transduction protein, molecular chaperone, cell skeleton proteins are identified, in which 3 proteins are down-regulated and 10 proteins are up-regulated. They might be correlated with the cause of PE.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Placenta
/
Preeclampsia
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Proteínas Gestacionales
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Perfilación de la Expresión Génica
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Proteómica
Límite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Anal Chim Acta
Año:
2008
Tipo del documento:
Article
País de afiliación:
China