13C NMR spectroscopic analysis of phospholipid metabolism in adrenal chromaffin cells.
NMR Biomed
; 4(3): 133-6, 1991 Jun.
Article
en En
| MEDLINE
| ID: mdl-1911100
ABSTRACT
Phosphatidylcholine (PC) metabolism in bovine adrenal chromaffin cells has been studied in vivo with 100 MHz 13C NMR spectroscopy. The incorporation of 13[CH3]choline was examined in cells embedded in 1% agarose strands and perfused at 37 degrees C. The pattern of 13C NMR spectroscopy. The incorporation of [13CH3]choline incorporation into PC of suspension cultures of chromaffin cells. The chemical shifts of labelled carbons in [13CH3]choline before and after their incorporation into PC are identical (ca 56.4 ppm). Incorporated [13CH3]choline can be released from the cells by phospholipase D which reduces the cellular PC signal by greater than 85%, indicating that a major fraction of the labelled PC is on the external membrane surface. This application of NMR spectroscopy provides a basis for direct measurement of the in vivo metabolism of PC in functioning adrenal chromaffin cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Gránulos Cromafines
/
Glándulas Suprarrenales
Límite:
Animals
Idioma:
En
Revista:
NMR Biomed
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
/
MEDICINA NUCLEAR
Año:
1991
Tipo del documento:
Article