[Intravesical perfusion of recombinant adeno-associated virus-endostatin in treatment of bladder cancer: experiments with mice].
Zhonghua Yi Xue Za Zhi
; 88(44): 3157-8, 2008 Dec 02.
Article
en Zh
| MEDLINE
| ID: mdl-19159603
ABSTRACT
OBJECTIVE:
To study the anti-tumor effect of intravesical perfusion of recombinant adeno-associated virus-endostatin (rAAV-ES) in treatment of bladder cancer.METHODS:
Forty-five C57BL/6 mice underwent intravesical perfusion of mouse bladder cancer cells of the line MB49 so as to establish orthotopic murine bladder cancer models and were divided into 3 equal groups, 3 days later to undergo intravesical perfusion of rAAV-ES, rAAV-EYFP, and PBS respectively once per week for 6 times. The anti-tumor effect of rAAV-ES on the tumor bearing mice was studied.RESULTS:
The tumor weight of the rAAV-ES group was (145 +/- 30) mg, significantly lighter than those of the rAAV-EYFP and PBS groups [(250 +/- 32) mg and (250 +/- 30) mg respectively, both P < 0.05]. The survival time of the rAAV-ES-treated mice was (46 +/- 7) d, significantly longer than those of the rAAV-EYFP- and PBS-treated groups [(38 +/- 7) d and (38 +/- 6) d respectively, both P < 0.05].CONCLUSION:
An effective biologic agent in bladder cancer gene therapy, intravesical treatment with rAAV-ES inhibits the angiogenesis, thus inhibiting the tumor formation and progression.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Terapia Genética
/
Dependovirus
/
Endostatinas
/
Neoplasias Experimentales
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Año:
2008
Tipo del documento:
Article
País de afiliación:
China