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Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis.
Masuda, Shinji; Ikeda, Rei; Masuda, Tatsuru; Hashimoto, Haruki; Tsuchiya, Tohru; Kojima, Hiroko; Nomata, Jiro; Fujita, Yuichi; Mimuro, Mamoru; Ohta, Hiroyuki; Takamiya, Ken-Ichiro.
Afiliación
  • Masuda S; Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. shmasuda@bio.titech.ac.jp
Plant J ; 58(6): 952-60, 2009 Jun.
Article en En | MEDLINE | ID: mdl-19222806
ABSTRACT
In angiosperms, chlorophyll biosynthesis is light dependent. A key factor in this process is protochlorophyllide oxidoreductase (POR), which requires light to catalyze the reduction of protochlorophyllide to chlorophyllide. It is believed that this protein originated from an ancient cyanobacterial enzyme that was introduced into proto-plant cells during the primary symbiosis. Here we report that PORs from the cyanobacteria Gloeobacter violaceus PCC7421 and Synechocystis sp. PCC6803 function in plastids. First, we found that the G. violaceus POR shows a higher affinity to its substrate protochlorophyllide than the Synechocystis POR but a similar affinity to plant PORs. Secondly, the reduced size of prolamellar bodies caused by a knockdown mutation of one of the POR genes, PORA, in Arabidopsis could be complemented by heterologous expression of the cyanobacterial PORs. Photoactive protochlorophyllide in the etioplasts of the complementing lines, however, was retained at a low level as in the parent PORA knockdown mutant, indicating that the observed formation of prolamellar bodies was irrelevant to the assembly of photoactive protochlorophyllide. This work reveals a new view on the formation of prolamellar bodies and provides new clues about the function of POR in the etioplast-chloroplast transition.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Protoclorofilida / Arabidopsis / Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH / Synechocystis Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2009 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Protoclorofilida / Arabidopsis / Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH / Synechocystis Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2009 Tipo del documento: Article País de afiliación: Japón