Assay for neural induction in the chick embryo.
J Vis Exp
; (24)2009 Feb 13.
Article
en En
| MEDLINE
| ID: mdl-19229186
ABSTRACT
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host competent ectoderm. The organizer graft instructs overlying na ve tissue to adopt a neural fate via neural inducing signals. This mechanism is referred to as neural induction, and constitutes the initial step in the formation of brain and spinal cord in amniotes. This method is essentially used for the characterization of putative neural inducing molecules in chick. This video demonstrates the different steps in the assay for neural induction; First, the donnor embryo is explanted and pinned on a dish. Then, the host embryo is prepared for New culture. The graft is excised and transplanted to the host area pellucida margin. The host is cultured for 18-22 hrs. The assembly is fixed and processed for further applications (e.g. in situ hybridization). This method was originally devised by Waddington (1,2) and Gallera (3,4).
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Embrión de Pollo
/
Técnicas de Cultivo de Embriones
/
Sistema Nervioso
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos