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Deregulation of DSE1 gene expression results in aberrant budding within the birth scar and cell wall integrity pathway activation in Saccharomyces cerevisiae.
Frydlová, Ivana; Malcová, Ivana; Vasicová, Pavla; Hasek, Jirí.
Afiliación
  • Frydlová I; Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Eukaryot Cell ; 8(4): 586-94, 2009 Apr.
Article en En | MEDLINE | ID: mdl-19252124
ABSTRACT
Strains of Saccharomyces cerevisiae lacking Isw2, the catalytic subunit of the Isw2 chromatin remodeling complex, show the mating type-independent activation of the cell wall integrity (CWI) signaling pathway. Since the CWI pathway activation usually reflects cell wall defects, we searched for the cell wall-related genes changed in expression. The genes DSE1, CTS1, and CHS1 were upregulated as a result of the absence of Isw2, according to previously published gene expression profiles (I. Frydlova, M. Basler, P. Vasicova, I. Malcova, and J. Hasek, Curr. Genet. 5287-95, 2007). Western blot analyses of double deletion mutants, however, did not indicate the contribution of the chitin metabolism-related genes CTS1 and CHS1 to the CWI pathway activation. Nevertheless, the deletion of the DSE1 gene encoding a daughter cell-specific protein with unknown function suppressed CWI pathway activation in isw2Delta cells. In addition, the deletion of DSE1 also abolished the budding-within-the-birth-scar phenotype of isw2Delta cells. The plasmid-driven overexpression proved that the deregulation of Dse1 synthesis was also responsible for CWI pathway activation and manifestation of the budding-within-the-birth-scar phenotype in wild-type cells. The overproduced Dse1-green fluorescent protein localized to both sides of the septum and persisted in unbudded cells. Although the exact cellular role of this daughter cell-specific protein has to be elucidated, our data point to the involvement of Dse1 in bud site selection in haploid cells.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Regulación Fúngica de la Expresión Génica / División Celular / Pared Celular Idioma: En Revista: Eukaryot Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2009 Tipo del documento: Article País de afiliación: República Checa

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Regulación Fúngica de la Expresión Génica / División Celular / Pared Celular Idioma: En Revista: Eukaryot Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2009 Tipo del documento: Article País de afiliación: República Checa