Velocity, processivity, and individual steps of single myosin V molecules in live cells.
Biophys J
; 96(10): 4268-75, 2009 May 20.
Article
en En
| MEDLINE
| ID: mdl-19450497
ABSTRACT
We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Miosina Tipo V
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biophys J
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia