Identification of FAM46D as a novel cancer/testis antigen using EST data and serological analysis.
Genomics
; 94(3): 153-60, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19540335
ABSTRACT
Cancer/testis Antigens (CTAs) are immunogenic proteins with a restricted expression pattern in normal tissues and aberrant expression in different types of tumors being considered promising candidates for immunotherapy. We used the alignment between EST sequences and the human genome sequence to identify novel CT genes. By examining the EST tissue composition of known CT clusters we defined parameters for the selection of 1184 EST clusters corresponding to putative CT genes. The expression pattern of 70 CT gene candidates was evaluated by RT-PCR in 21 normal tissues, 17 tumor cell lines and 160 primary tumors. We were able to identify 4 CT genes expressed in different types of tumors. The presence of antibodies against the protein encoded by 1 of these 4 CT genes (FAM46D) was exclusively detected in plasma samples from cancer patients. Due to its restricted expression pattern and immunogenicity FAM46D represents a novel target for cancer immunotherapy.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Etiquetas de Secuencia Expresada
/
Antígenos de Neoplasias
/
Proteínas de Neoplasias
/
Neoplasias
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Brasil