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A UVB-hypersensitive mutant in Arabidopsis thaliana is defective in the DNA damage response.
Sakamoto, Ayako N; Lan, Vo Thi Thuong; Puripunyavanich, Vichai; Hase, Yoshihiro; Yokota, Yuichiro; Shikazono, Naoya; Nakagawa, Mayu; Narumi, Issay; Tanaka, Atsushi.
Afiliación
  • Sakamoto AN; Radiation-Applied Biology Division, Japan Atomic Energy Agency, Watanuki-machi 1233, Takasaki, Gumma 370-1292, Japan. sakamoto.ayako@jaea.go.jp
Plant J ; 60(3): 509-17, 2009 Nov.
Article en En | MEDLINE | ID: mdl-19619159
To investigate UVB DNA damage response in higher plants, we used a genetic screen to isolate Arabidopsis thaliana mutants that are hypersensitive to UVB irradiation, and isolated a UVB-sensitive mutant, termed suv2 (for sensitive to UV 2) that also displayed hypersensitivity to gamma-radiation and hydroxyurea. This phenotype is reminiscent of the Arabidopsis DNA damage-response mutant atr. The suv2 mutation was mapped to the bottom of chromosome 5, and contains an insertion in an unknown gene annotated as MRA19.1. RT-PCR analysis with specific primers to MRA19.1 detected a transcript consisting of 12 exons. The transcript is predicted to encode a 646 amino acid protein that contains a coiled-coil domain and two instances of predicted PIKK target sequences within the N-terminal region. Fusion proteins consisting of the predicted MRA19.1 and DNA-binding or activation domain of yeast transcription factor GAL4 interacted with each other in a yeast two-hybrid system, suggesting that the proteins form a homodimer. Expression of CYCB1;1:GUS gene, which encodes a labile cyclin:GUS fusion protein to monitor mitotic activity by GUS activity, was weaker in the suv2 plant after gamma-irradiation than in the wild-type plants and was similar to that in the atr plants, suggesting that the suv2 mutant is defective in cell-cycle arrest in response to DNA damage. Overall, these results suggest that the gene disrupted in the suv2 mutant encodes an Arabidopsis homologue of the ATR-interacting protein ATRIP.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Daño del ADN / Arabidopsis / ADN de Plantas / Mutación Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2009 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Daño del ADN / Arabidopsis / ADN de Plantas / Mutación Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2009 Tipo del documento: Article País de afiliación: Japón