Optimization of the detection of microbes in blood from immunocompromised patients with haematological malignancies.
Clin Microbiol Infect
; 15(7): 680-3, 2009 Jul.
Article
en En
| MEDLINE
| ID: mdl-19624509
ABSTRACT
The present study aimed to improve the rate of detection of blood-borne microbes by using PCRs with pan-bacterial and Candida specificity. Seventeen per cent of the blood samples (n=178) collected from 107 febrile patients with haematological malignancies were positive using standard culture (BacT/Alert system). Candida PCR was positive in 12 patients, only one of whom scored culture-positive. Bacterial PCR using fresh blood samples was often negative, but the detection rate increased when the blood was pre-incubated for 2 days. These data indicate that PCR assays might be a complement for the detection of blood-borne opportunists in immunocompromised haematology patients.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sangre
/
Candida
/
Reacción en Cadena de la Polimerasa
/
Fungemia
/
Bacteriemia
/
Neoplasias Hematológicas
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Microbiol Infect
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Suecia