Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR.
Water Res
; 43(19): 4918-28, 2009 Nov.
Article
en En
| MEDLINE
| ID: mdl-19631959
ABSTRACT
The prevalence and concentrations of Campylobacter jejuni, Salmonella spp. and enterohaemorrhagic Escherichia coli (EHEC) were investigated in surface waters in Brisbane, Australia using quantitative PCR (qPCR) based methodologies. Water samples were collected from Brisbane City Botanic Gardens (CBG) Pond, and two urban tidal creeks (i.e., Oxley Creek and Blunder Creek). Of the 32 water samples collected, 8 (25%), 1 (3%), 9 (28%), 14 (44%), and 15 (47%) were positive for C. jejuni mapA, Salmonella invA, EHEC O157 LPS, EHEC VT1, and EHEC VT2 genes, respectively. The presence/absence of the potential pathogens did not correlate with either E. coli or enterococci concentrations as determined by binary logistic regression. In conclusion, the high prevalence, and concentrations of potential zoonotic pathogens along with the concentrations of one or more fecal indicators in surface water samples indicate a poor level of microbial quality of surface water, and could represent a significant health risk to users. The results from the current study would provide valuable information to the water quality managers in terms of minimizing the risk from pathogens in surface waters.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Salmonella
/
Contaminantes del Agua
/
Monitoreo del Ambiente
/
Campylobacter jejuni
/
Escherichia coli
/
Agua Dulce
Tipo de estudio:
Prevalence_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Water Res
Año:
2009
Tipo del documento:
Article
País de afiliación:
Australia