Reversed-phase liquid chromatographic profile of free amino acids in strawberry-tree (Arbutus unedo L.) honey.
J AOAC Int
; 92(4): 1145-52, 2009.
Article
en En
| MEDLINE
| ID: mdl-19714983
ABSTRACT
The typical profile of the free amino acids contained in strawberry-tree (Arbutus unedo L.) honey is reported for the first time. An optimized reversed-phase liquid chromatographic (RP-LC) method with phenyl isothiocyanate precolumn derivatization was used. Fourteen free amino acids were identified and quantified in 16 analytical samples. Proline (65.63%) was found to be the most abundant free amino acid, followed by glutamic acid (6.49%), arginine (5.21%), alanine (5.17%), and phenylalanine (4.97%). The total free amino acid content of strawberry-tree honey (average value, 436 mg/kg) was found to be low in comparison to amounts cited in the literature concerning unifloral honeys. The analytical method was optimized and fully validated in terms of detection and quantitation limits, precision (by testing repeatability and reproducibility), linearity, and bias (by means of recovery tests). The acceptability of the validation protocol results was verified using Horwitz's mathematical model and AOAC guidelines.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ericaceae
/
Aminoácidos
/
Miel
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
Europa
Idioma:
En
Revista:
J AOAC Int
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia