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Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.
Kmieciak, Maciej; Gowda, Madhu; Graham, Laura; Godder, Kamar; Bear, Harry D; Marincola, Francesco M; Manjili, Masoud H.
Afiliación
  • Kmieciak M; Department of Microbiology & Immunology, Virginia Commonwealth University Massey Cancer Center, Richmond, USA. mkmieciak@vcu.edu
J Transl Med ; 7: 89, 2009 Oct 22.
Article en En | MEDLINE | ID: mdl-19849846
ABSTRACT

BACKGROUND:

Foxp3 has been suggested to be a standard marker for murine Tregs whereas its role as marker for human Tregs is controversial. While some reports have shown that human Foxp3+ T cells had no regulatory function others have shown their role in the inhibition of T cell proliferation.

METHODS:

T cell activation was performed by means of brayostatin-1/ionomycin (B/I), mixed lymphocyte reaction (MLR), and CD3/CD28 activation. T cell proliferation was performed using BrdU and CFSE staining. Flow cytometry was performed to determine Foxp3 expression, cell proliferation, viabilities and phenotype analyses of T cells.

RESULTS:

Both CD4+ and CD8+ T cells expressed Foxp3 upon activation in vitro. Expression of Foxp3 remained more stable in CD4+CD25+ T cells compared to that in CD8+CD25+ T cells. The CD4+CD25+Foxp3+ T cells expressed CD44 and CD62L, showing their effector and memory phenotypes. Both FoxP3- responder T cells and CD4+FoxP3+ T cells underwent proliferation upon CD3/CD28 activation.

CONCLUSION:

Expression of Foxp3 does not necessarily convey regulatory function in human CD4+CD25+ T cells. Increased FoxP3 on CD44+ effector and CD44+CD62L+ memory T cells upon stimulation suggest the activation-induced regulation of FoxP3 expression.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Activación de Linfocitos / Subgrupos de Linfocitos T / Linfocitos T Reguladores / Factores de Transcripción Forkhead / Subunidad alfa del Receptor de Interleucina-2 Límite: Animals / Humans Idioma: En Revista: J Transl Med Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Activación de Linfocitos / Subgrupos de Linfocitos T / Linfocitos T Reguladores / Factores de Transcripción Forkhead / Subunidad alfa del Receptor de Interleucina-2 Límite: Animals / Humans Idioma: En Revista: J Transl Med Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos