[Multiplex fluorescent quantitative PCR for detecting deep fungal infection in patients with systemic lupus erythematosus].
Nan Fang Yi Ke Da Xue Xue Bao
; 29(10): 2112-4, 2121, 2009 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-19861279
ABSTRACT
OBJECTIVE:
To establish a rapid, sensitive and specific method based on multiplex fluorescent quantitative PCR for detection of deep infections with Candida albicans and Aspergillus flavus in patients with systemic lupus erythematosus (SLE).METHODS:
Two pairs of primers and Taqman probes were designed according to the gene sequences of Candida albicans and Aspergillus flavus available in American Type Culture Collection. The positivity rate, sensitivity and specificity of the multiplex fluorescent quantitative PCR-based method for detecting the fungal infection was tested in 20 specimens from SLE patients with Candida albicans and Aspergillus flavus infections, 20 specimens from SLE patients with suspected deep fungal infections, and 20 microbial samples other than Candida albicans or Aspergillus flavus.RESULTS:
The multiple fluorescence quantitative PCR-based method showed a positivity rate and specificity of both 100% for detecting Candida albicans and Aspergillus flavus infections in the SLE patients. This method resulted in a detection sensitivity of 75%, significantly higher than that of fugal culture method (40%, P<0.05).CONCLUSIONS:
The multiplex fluorescent real-time PCR-based method allows rapid, quantitative and simultaneous detection of deep Candida albicans and Aspergillus flavus infections with high sensitivity and specificity in SLE patients.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aspergillus
/
Candida albicans
/
Lupus Eritematoso Sistémico
/
Micosis
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Female
/
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2009
Tipo del documento:
Article
País de afiliación:
China