Application of glutaraldehyde to in-cell Western assay for normalization.
Anal Biochem
; 398(2): 254-6, 2010 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-20018162
ABSTRACT
Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnica del Anticuerpo Fluorescente
/
Glutaral
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2010
Tipo del documento:
Article
País de afiliación:
China