Distinct protein domains and expression patterns confer divergent axon guidance functions for Drosophila Robo receptors.
Cell
; 140(3): 409-20, 2010 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-20144763
ABSTRACT
The orthogonal array of axon pathways in the Drosophila CNS is constructed in part under the control of three Robo family axon guidance receptors Robo1, Robo2 and Robo3. Each of these receptors is responsible for a distinct set of guidance decisions. To determine the molecular basis for these functional specializations, we used homologous recombination to create a series of 9 "robo swap" alleles expressing each of the three Robo receptors from each of the three robo loci. We demonstrate that the lateral positioning of longitudinal axon pathways relies primarily on differences in gene regulation, not distinct combinations of Robo proteins as previously thought. In contrast, specific features of the Robo1 and Robo2 proteins contribute to their distinct functions in commissure formation. These specializations allow Robo1 to prevent crossing and Robo2 to promote crossing. These data demonstrate how diversification of expression and structure within a single family of guidance receptors can shape complex patterns of neuronal wiring.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Receptores Inmunológicos
/
Regulación del Desarrollo de la Expresión Génica
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Proteínas de Drosophila
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Drosophila melanogaster
/
Proteínas del Tejido Nervioso
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
2010
Tipo del documento:
Article
País de afiliación:
Austria